› 1eqj

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF PHOSPHOGLYCERATE MUTASE FROM BACILLUS STEAROTHERMOPHILUS COMPLEXED WITH 2-PHOSPHOGLYCERATE

Released:
DOI: 10.2210/pdb1eqj/pdb
PDB entry 1eqj coloured by chain, front view.
PDB entry 1eqj coloured by chain, side view.
PDB entry 1eqj coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus. Crystal structure of the complex with 2-phosphoglycerate.
Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G
J Biol Chem 275 23146-53 (2000)
PMID: 10764795

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = 3-phospho-D-glycerate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-bisphosphoglycerate-independent phosphoglycerate mutase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 57.02 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q9X519 (Residues: 1-511; Coverage: 100%)
Gene names: gpmI, pgm
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand 2PG 1 x 2PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 58.407Å b: 205.688Å c: 123.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.258
Expression system: Bacillus subtilis

Quick links

Citations

1 review citation

The Prodigal Compound: Return of Ribosyl 1,5-Bisphosphate as an Important Player in Metabolism.
Hove-Jensen et al. (2019)
27 other articles

2 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
1 more