› 1es8

X-ray diffraction
2.3Å resolution

Crystal structure of free BglII

Released:
DOI: 10.2210/pdb1es8/pdb
PDB entry 1es8 coloured by chain, front view.
PDB entry 1es8 coloured by chain, side view.
PDB entry 1es8 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease.
Lukacs CM, Kucera R, Schildkraut I, Aggarwal AK
Nat Struct Biol 8 126-30 (2001)
PMID: 11175900

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme BglII Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 26.03 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45488 (Residues: 1-223; Coverage: 100%)
Gene name: bglIIR
Structure domains: Restriction Endonuclease

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 79.15Å b: 79.15Å c: 66.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.258
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
1 more

4 mentions without citation

Origin of neointimal endothelium and alpha-actin-positive smooth muscle cells in transplant arteriosclerosis.
Hillebrands et al. (2001)
3 more