› 1esj

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF THIAZOLE KINASE MUTANT (C198S)

Released:
DOI: 10.2210/pdb1esj/pdb
PDB entry 1esj coloured by chain, front view.
PDB entry 1esj coloured by chain, side view.
PDB entry 1esj coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Campobasso N, Mathews II, Begley TP, Ealick SE
Biochemistry 39 7868-77 (2000)
PMID: 10891066

Function and Biology Details

Reaction catalysed: 
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyethylthiazole kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 284 amino acids
Theoretical weight: 29.63 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39593 (Residues: 1-272; Coverage: 100%)
Gene names: BSU38300, ipa-25d, thiK, thiM, ywbJ
Sequence domains: Hydroxyethylthiazole kinase family
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 53.06Å b: 100.89Å c: 73.07Å
α: 90° β: 96.02° γ: 90°
R-values:
R R work R free
0.231 0.229 0.253
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The structural and biochemical foundations of thiamin biosynthesis.
Jurgenson et al. (2009)
2 more

1 mention without citation

New structural insights and molecular-modelling studies of 4-methyl-5-beta-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK).
Jeyakanthan et al. (2009)