› 1ess

Solution NMR

STEROID TETHERED DNA, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1ess/pdb
PDB entry 1ess coloured by chain, front view.
PDB entry 1ess coloured by chain, side view.
PDB entry 1ess coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3').
Denisov AY, Sandström A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J
J Biomol Struct Dyn 15 499-516 (1997)
PMID: 9439997

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113766 (preferred)
Entry contents:
3 distinct DNA molecules
Macromolecules (3 distinct):
DNA (5'-D(P*TP*GP*GP*AP*GP*CP*TP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 8 nucleotides
Theoretical weight: 2.48 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*AP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.18 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*CP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 1.15 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ESO 2 x ESO
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RESTRAINED MD
Expression system: Not provided

Quick links

Citations

1 citations in other articles

NMR structure of an ethylene interstrand cross-linked DNA which mimics the lesion formed by 1,3-bis(2-chloroethyl)-1-nitrosourea.
O'Flaherty et al. (2014)