› 1est

X-ray diffraction
2.5Å resolution

THE ATOMIC STRUCTURE OF CRYSTALLINE PORCINE PANCREATIC ELASTASE AT 2.5 ANGSTROMS RESOLUTION. COMPARISONS WITH THE STRUCTURE OF ALPHA-CHYMOTRYPSIN

Released:
DOI: 10.2210/pdb1est/pdb
PDB entry 1est coloured by chain, front view.
PDB entry 1est coloured by chain, side view.
PDB entry 1est coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
The atomic structure of crystalline porcine pancreatic elastase at 2.5 A resolution: comparisons with the structure of alpha-chymotrypsin.
Sawyer L, Shotton DM, Campbell JW, Wendell PL, Muirhead H, Watson HC
J Mol Biol 118 137-208 (1978)
PMID: 628010

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin-like elastase family member 1 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand TSU 1 x TSU
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 51.5Å b: 58Å c: 75.5Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

9 review citations

Hydrogen bonding in globular proteins.
Baker et al. (1984)
8 more

6 mentions without citation

Biophysical highlights from 54 years of macromolecular crystallography.
Richardson et al. (2014)
5 more