› 1ev7

X-ray diffraction
2.38Å resolution

CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI

Released:
DOI: 10.2210/pdb1ev7/pdb
PDB entry 1ev7 coloured by chain, front view.
PDB entry 1ev7 coloured by chain, side view.
PDB entry 1ev7 coloured by chain, top view.
Source organism: Lentzea aerocolonigenes
Primary publication:
Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase.
Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H
EMBO J 19 3110-8 (2000)
PMID: 10856254

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme NaeI Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.38 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P50187 (Residues: 1-317; Coverage: 100%)
Gene name: naeIR
Sequence domains: Restriction endonuclease NaeI
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 99.78Å b: 56.16Å c: 59.05Å
α: 90° β: 95.92° γ: 90°
R-values:
R R work R free
0.238 0.238 0.287
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
6 more

5 mentions without citation

Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase.
Huai et al. (2000)
4 more