› 1ew3

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1

Released:
DOI: 10.2210/pdb1ew3/pdb
PDB entry 1ew3 coloured by chain, front view.
PDB entry 1ew3 coloured by chain, side view.
PDB entry 1ew3 coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Crystal structure of the allergen Equ c 1. A dimeric lipocalin with restricted IgE-reactive epitopes.
Lascombe MB, Grégoire C, Poncet P, Tavares GA, Rosinski-Chupin I, Rabillon J, Goubran-Botros H, Mazié JC, David B, Alzari PM
J Biol Chem 275 21572-7 (2000)
PMID: 10787420

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major allergen Equ c 1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.75 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95182 (Residues: 23-181; Coverage: 92%)
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P41212
Unit cell:
a: 84.04Å b: 84.04Å c: 58.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.252
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Recombinant allergens for diagnosis and therapy of allergic disease.
Chapman et al. (2000)
9 more

16 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
15 more