› 1ewk

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF METABOTROPIC GLUTAMATE RECEPTOR SUBTYPE 1 COMPLEXED WITH GLUTAMATE

Released:
DOI: 10.2210/pdb1ewk/pdb
PDB entry 1ewk coloured by chain, front view.
PDB entry 1ewk coloured by chain, side view.
PDB entry 1ewk coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor.
Kunishima N, Shimada Y, Tsuji Y, Sato T, Yamamoto M, Kumasaka T, Nakanishi S, Jingami H, Morikawa K
Nature 407 971-7 (2000)
PMID: 11069170

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metabotropic glutamate receptor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 55.26 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23385 (Residues: 33-522; Coverage: 42%)
Gene names: Gprc1a, Grm1, Mglur1
Sequence domains: Receptor family ligand binding region
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand NAG 4 x NAG
Ligand MG 2 x MG
Ligand GLU 2 x GLU
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 83.375Å b: 95.219Å c: 97.451Å
α: 90° β: 114.85° γ: 90°
R-values:
R R work R free
0.198 0.196 0.227
Expression system: Spodoptera frugiperda

Quick links

Citations

208 review citations

Glutamate receptor ion channels: structure, regulation, and function.
Traynelis et al. (2010)
207 more

77 mentions without citation

Metabotropic glutamate receptors: from the workbench to the bedside.
Nicoletti et al. (2011)
76 more