› 1ewq

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE TAQ MUTS COMPLEXED WITH A HETERODUPLEX DNA AT 2.2 A RESOLUTION

Released:
DOI: 10.2210/pdb1ewq/pdb
PDB entry 1ewq coloured by chain, front view.
PDB entry 1ewq coloured by chain, side view.
PDB entry 1ewq coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA.
Obmolova G, Ban C, Hsieh P, Yang W
Nature 407 703-10 (2000)
PMID: 11048710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113775 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 765 amino acids
Theoretical weight: 86.39 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56215 (Residues: 1-765; Coverage: 94%)
Gene name: mutS
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*CP*GP*CP*TP*AP*GP*CP*GP*TP*GP*CP*GP*GP*CP*TP*CP*GP*TP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.07 KDa
Source organism: Thermus aquaticus
Expression system: Not provided
DNA (5'-D(*GP*GP*AP*CP*GP*AP*GP*CP*CP*GP*CP*CP*GP*CP*TP*AP*GP*CP*GP*TP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.78 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand EDO 7 x EDO
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 103.167Å b: 114.216Å c: 160.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

98 review citations

Genome maintenance mechanisms for preventing cancer.
Hoeijmakers JH. (2001)
97 more

26 mentions without citation

Single-molecule views of MutS on mismatched DNA.
Lee et al. (2014)
25 more