› 1ewr

X-ray diffraction
3.19Å resolution

CRYSTAL STRUCTURE OF TAQ MUTS

Released:
DOI: 10.2210/pdb1ewr/pdb
PDB entry 1ewr coloured by chain, front view.
PDB entry 1ewr coloured by chain, side view.
PDB entry 1ewr coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA.
Obmolova G, Ban C, Hsieh P, Yang W
Nature 407 703-10 (2000)
PMID: 11048710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 649 amino acids
Theoretical weight: 73.05 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56215 (Residues: 117-765; Coverage: 80%)
Gene name: mutS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 96.734Å b: 96.734Å c: 427.126Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.331 0.331 0.361
Expression system: Escherichia coli

Quick links

Citations

97 review citations

Genome maintenance mechanisms for preventing cancer.
Hoeijmakers JH. (2001)
96 more

11 mentions without citation

Single molecule studies of DNA mismatch repair.
Erie et al. (2014)
10 more