› 1ex6

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF UNLIGANDED FORM OF GUANYLATE KINASE FROM YEAST

Released:
DOI: 10.2210/pdb1ex6/pdb
PDB entry 1ex6 coloured by chain, front view.
PDB entry 1ex6 coloured by chain, side view.
PDB entry 1ex6 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of unligated guanylate kinase from yeast reveals GMP-induced conformational changes.
Blaszczyk J, Li Y, Yan H, Ji X
J Mol Biol 307 247-57 (2001)
PMID: 11243817

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 20.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15454 (Residues: 2-187; Coverage: 100%)
Gene names: D9461.39, GUK1, YDR454C
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P31
Unit cell:
a: 51.559Å b: 51.559Å c: 119.645Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.175 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The ß subunit of voltage-gated Ca2+ channels.
Buraei et al. (2010)
2 more

18 mentions without citation

Conformational transitions upon ligand binding: holo-structure prediction from apo conformations.
Seeliger et al. (2010)
17 more