› 1ex9

X-ray diffraction
2.54Å resolution

CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE

Released:
DOI: 10.2210/pdb1ex9/pdb
PDB entry 1ex9 coloured by chain, front view.
PDB entry 1ex9 coloured by chain, side view.
PDB entry 1ex9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases.
Nardini M, Lang DA, Liebeton K, Jaeger KE, Dijkstra BW
J Biol Chem 275 31219-25 (2000)
PMID: 10893416

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 30.16 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: P26876 (Residues: 27-311; Coverage: 100%)
Gene names: PA2862, lip, lipA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand OCP 1 x OCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 45.47Å b: 50.963Å c: 110.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.245
Expression system: Pseudomonas aeruginosa

Quick links

Citations

12 review citations

The biofilm matrix.
Flemming et al. (2010)
11 more

25 mentions without citation

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
24 more