› 1eyb

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF APO HUMAN HOMOGENTISATE DIOXYGENASE

Released:
DOI: 10.2210/pdb1eyb/pdb
PDB entry 1eyb coloured by chain, front view.
PDB entry 1eyb coloured by chain, side view.
PDB entry 1eyb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human homogentisate dioxygenase.
Titus GP, Mueller HA, Burgner J, Rodríguez De Córdoba S, Peñalva MA, Timm DE
Nat Struct Biol 7 542-6 (2000)
PMID: 10876237

Function and Biology Details

Reaction catalysed: 
Homogentisate + O(2) = 4-maleylacetoacetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homogentisate 1,2-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 53.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93099 (Residues: 1-445; Coverage: 100%)
Gene names: HGD, HGO
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P6322
Unit cell:
a: 158.38Å b: 158.38Å c: 93.459Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.222
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The 2-His-1-carboxylate facial triad: a versatile platform for dioxygen activation by mononuclear non-heme iron(II) enzymes.
Koehntop et al. (2005)
12 more

8 mentions without citation

Edgetic perturbation models of human inherited disorders.
Zhong et al. (2009)
7 more