1eyg

X-ray diffraction
2.8Å resolution

Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS

Released:
Source organism: Escherichia coli
Primary publication:
Structure of the DNA binding domain of E. coli SSB bound to ssDNA.
Nat Struct Biol 7 648-52 (2000)
PMID: 10932248

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-113779 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Single-stranded DNA-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 116 amino acids
Theoretical weight: 12.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGE0 (Residues: 1-116; Coverage: 65%)
Gene names: JW4020, b4059, exrB, lexC, ssb
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins
SINGLE STRANDED 28-MER OF D(C) Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 35 nucleotides
Theoretical weight: 10.08 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 98.688Å b: 71.081Å c: 79.16Å
α: 90° β: 91.93° γ: 90°
R-values:
R R work R free
0.256 0.256 0.298
Expression systems:
  • Escherichia coli
  • Not provided