› 1ezj

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE MULTIMERIZATION DOMAIN OF THE PHOSPHOPROTEIN FROM SENDAI VIRUS

Released:
DOI: 10.2210/pdb1ezj/pdb
PDB entry 1ezj coloured by chain, front view.
PDB entry 1ezj coloured by chain, side view.
PDB entry 1ezj coloured by chain, top view.
Source organism: Sendai virus (strain Harris)
Primary publication:
Tetrameric coiled coil domain of Sendai virus phosphoprotein.
Tarbouriech N, Curran J, Ruigrok RW, Burmeister WP
Nat Struct Biol 7 777-81 (2000)
PMID: 10966649

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoprotein Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.12 KDa
Source organism: Sendai virus (strain Harris)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04859 (Residues: 320-433; Coverage: 20%)
Gene name: P/V/C
Sequence domains: Paramyxovirinae P phosphoprotein C-terminal region
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EMC 1 x EMC
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P4212
Unit cell:
a: 48.584Å b: 48.584Å c: 100.847Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.249 0.293
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

Viral DNA polymerase scanning and the gymnastics of Sendai virus RNA synthesis.
Kolakofsky et al. (2004)
16 more

11 mentions without citation

The Structure and Topology of α-Helical Coiled Coils.
Lupas et al. (2017)
10 more