› 1f02

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF C-TERMINAL 282-RESIDUE FRAGMENT OF INTIMIN IN COMPLEX WITH TRANSLOCATED INTIMIN RECEPTOR (TIR) INTIMIN-BINDING DOMAIN

Released:
DOI: 10.2210/pdb1f02/pdb
PDB entry 1f02 coloured by chain, front view.
PDB entry 1f02 coloured by chain, side view.
PDB entry 1f02 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of enteropathogenic Escherichia coli intimin-receptor complex.
Luo Y, Frey EA, Pfuetzner RA, Creagh AL, Knoechel DG, Haynes CA, Finlay BB, Strynadka NC
Nature 405 1073-7 (2000)
PMID: 10890451

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219340 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Intimin Chain: I
Molecule details ›
Chain: I
Length: 282 amino acids
Theoretical weight: 30.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19809 (Residues: 658-939; Coverage: 30%)
Gene names: E2348C_3939, eae, eaeA
Sequence domains:
Structure domains:
Translocated intimin receptor Tir Chain: T
Molecule details ›
Chain: T
Length: 66 amino acids
Theoretical weight: 7.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KWH9 (Residues: 272-336; Coverage: 12%)
Gene name: tir
Sequence domains: Translocated intimin receptor (Tir) intimin-binding domain
Structure domains: Translocated intimin receptor, central domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 90.5Å b: 349Å c: 47.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

51 review citations

The C-type lectin-like domain superfamily.
Zelensky et al. (2005)
50 more

15 mentions without citation

Neutralizing Antibodies Targeting the Conserved Stem Region of Influenza Hemagglutinin.
Nath Neerukonda et al. (2020)
14 more