› 1f0l

X-ray diffraction
1.55Å resolution

1.55 ANGSTROM CRYSTAL STRUCTURE OF WILD TYPE DIPHTHERIA TOXIN

Released:
DOI: 10.2210/pdb1f0l/pdb
PDB entry 1f0l coloured by chain, front view.
PDB entry 1f0l coloured by chain, side view.
PDB entry 1f0l coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Primary publication:
Characterization of High-Order Oligomerization and Energetics in Diphtheria Toxin
Steere B
Thesis (2001)

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphtheria toxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 58.41 KDa
Source organism: Corynebacterium diphtheriae
UniProt:
  • Canonical: P00588 (Residues: 33-567; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 9 x CL
Ligand APU 2 x APU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21212
Unit cell:
a: 167.77Å b: 134.88Å c: 46.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.238

Quick links

Citations

12 mentions without citation

Toxin-based therapeutic approaches.
Shapira et al. (2010)
11 more