› 1f0v

X-ray diffraction
1.7Å resolution

Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping

Released:
DOI: 10.2210/pdb1f0v/pdb
PDB entry 1f0v coloured by chain, front view.
PDB entry 1f0v coloured by chain, side view.
PDB entry 1f0v coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A domain-swapped RNase A dimer with implications for amyloid formation.
Liu Y, Gotte G, Libonati M, Eisenberg D
Nat Struct Biol 8 211-4 (2001)
PMID: 11224563

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113783 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ribonuclease pancreatic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
5'-D(*CP*G)-3' Chains: M, N, O, P
Molecule details ›
Chains: M, N, O, P
Length: 2 nucleotides
Theoretical weight: 573 Da
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand GOL 24 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 83.569Å b: 96.341Å c: 48.24Å
α: 90° β: 107.5° γ: 90°
R-values:
R R work R free
0.184 0.184 0.213
Expression system: Not provided

Quick links

Citations

31 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
30 more

16 mentions without citation

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
15 more