› 1f1t

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE MALACHITE GREEN APTAMER COMPLEXED WITH TETRAMETHYL-ROSAMINE

Released:
DOI: 10.2210/pdb1f1t/pdb
PDB entry 1f1t coloured by chain, front view.
PDB entry 1f1t coloured by chain, side view.
PDB entry 1f1t coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
2.8 A crystal structure of the malachite green aptamer.
Baugh C, Grate D, Wilson C
J Mol Biol 301 117-28 (2000)
PMID: 10926496

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196008 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
MALACHITE GREEN APTAMER RNA Chain: A
Molecule details ›
Chain: A
Length: 38 nucleotides
Theoretical weight: 12.71 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 8 x SR
Ligand ROS 1 x ROS
1 modified residue:
Ligand 5BU 5 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P6522
Unit cell:
a: 55.297Å b: 55.297Å c: 143.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.261 0.282
Expression system: Not provided

Quick links

Citations

21 review citations

Riboswitches as versatile gene control elements.
Tucker et al. (2005)
20 more

21 mentions without citation

Selection and Biosensor Application of Aptamers for Small Molecules.
Pfeiffer et al. (2016)
20 more