› 1f1w

X-ray diffraction
2.1Å resolution

SRC SH2 THREF1TRP MUTANT COMPLEXED WITH THE PHOSPHOPEPTIDE S(PTR)VNVQN

Released:
DOI: 10.2210/pdb1f1w/pdb
PDB entry 1f1w coloured by chain, front view.
PDB entry 1f1w coloured by chain, side view.
PDB entry 1f1w coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis for specificity switching of the Src SH2 domain.
Kimber MS, Nachman J, Cunningham AM, Gish GD, Pawson T, Pai EF
Mol Cell 5 1043-9 (2000)
PMID: 10911998

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-132631 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.06 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 144-247; Coverage: 20%)
Gene name: SRC
Sequence domains: SH2 domain
Structure domains: SH2 domain
S(PTR)VNVQN PHOSPHOPEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 903 Da
Source organism: Gallus gallus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: C2221
Unit cell:
a: 33.714Å b: 56.804Å c: 102.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Phosphotyrosine-binding domains in signal transduction.
Yaffe MB. (2002)
4 more

1 mention without citation

Genome-wide prediction of SH2 domain targets using structural information and the FoldX algorithm.
Sánchez et al. (2008)