› 1f1z

X-ray diffraction
2.4Å resolution

TNSA, a catalytic component of the TN7 transposition system

Released:
DOI: 10.2210/pdb1f1z/pdb
PDB entry 1f1z coloured by chain, front view.
PDB entry 1f1z coloured by chain, side view.
PDB entry 1f1z coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Unexpected structural diversity in DNA recombination: the restriction endonuclease connection.
Hickman AB, Li Y, Mathew SV, May EW, Craig NL, Dyda F
Mol Cell 5 1025-34 (2000)
PMID: 10911996

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transposon Tn7 transposition protein TnsA Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 31.3 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P13988 (Residues: 1-273; Coverage: 100%)
Gene name: tnsA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 38.35Å b: 83.68Å c: 97.94Å
α: 90° β: 94.07° γ: 90°
R-values:
R R work R free
0.205 0.202 0.254

Quick links

Citations

18 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
17 more

8 mentions without citation

The PD-(D/E)XK superfamily revisited: identification of new members among proteins involved in DNA metabolism and functional predictions for domains of (hitherto) unknown function.
Kosinski et al. (2005)
7 more