› 1f27

X-ray diffraction
1.3Å resolution

CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT

Released:
DOI: 10.2210/pdb1f27/pdb
PDB entry 1f27 coloured by chain, front view.
PDB entry 1f27 coloured by chain, side view.
PDB entry 1f27 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The 1.3 A crystal structure of a biotin-binding pseudoknot and the basis for RNA molecular recognition.
Nix J, Sussman D, Wilson C
J Mol Biol 296 1235-44 (2000)
PMID: 10698630

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196009 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*AP*CP*CP*GP*UP*CP*AP*GP*AP*GP*GP*AP*CP*AP*CP*GP*GP*UP*U)-3') Chain: A
Molecule details ›
Chain: A
Length: 19 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*AP*AP*AP*AP*AP*GP*UP*CP*CP*UP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.47 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand BTN 1 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 47.59Å b: 30.96Å c: 62.11Å
α: 90° β: 111.88° γ: 90°
R-values:
R R work R free
0.199 0.199 0.238
Expression system: Not provided

Quick links

Citations

9 review citations

SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands.
Stoltenburg et al. (2007)
8 more

10 mentions without citation

Selection and Biosensor Application of Aptamers for Small Molecules.
Pfeiffer et al. (2016)
9 more