› 1f3x

X-ray diffraction
2.8Å resolution

S402P MUTANT OF RABBIT MUSCLE PYRUVATE KINASE

Released:
DOI: 10.2210/pdb1f3x/pdb
PDB entry 1f3x coloured by chain, front view.
PDB entry 1f3x coloured by chain, side view.
PDB entry 1f3x coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural and functional linkages between subunit interfaces in mammalian pyruvate kinase.
Wooll JO, Friesen RH, White MA, Watowich SJ, Fox RO, Lee JC, Czerwinski EW
J Mol Biol 312 525-40 (2001)
PMID: 11563914

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 530 amino acids
Theoretical weight: 57.99 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P11974 (Residues: 2-531; Coverage: 100%)
Gene name: PKM
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand K 8 x K
Ligand MN 8 x MN
Ligand PYR 8 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P1
Unit cell:
a: 82.138Å b: 108.707Å c: 144.387Å
α: 95.26° β: 93.47° γ: 112.24°
R-values:
R R work R free
0.241 0.241 0.27
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Modulation of allostery of pyruvate kinase by shifting of an ensemble of microstates.
Lee JC. (2008)
28 other articles

1 mention without citation

Rapid catalytic template searching as an enzyme function prediction procedure.
Nilmeier et al. (2013)