› 1f42

X-ray diffraction
2.5Å resolution

THE P40 DOMAIN OF HUMAN INTERLEUKIN-12

Released:
DOI: 10.2210/pdb1f42/pdb
PDB entry 1f42 coloured by chain, front view.
PDB entry 1f42 coloured by chain, side view.
PDB entry 1f42 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Charged residues dominate a unique interlocking topography in the heterodimeric cytokine interleukin-12.
Yoon C, Johnston SC, Tang J, Stahl M, Tobin JF, Somers WS
EMBO J 19 3530-41 (2000)
PMID: 10899108

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
1 bound ligand:
Ligand MNB 2 x MNB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 34Å b: 55Å c: 189.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.227 0.281
Expression system: Cricetulus griseus

Quick links

Citations

22 review citations

IL-12 family cytokines: immunological playmakers.
Vignali et al. (2012)
21 more

6 mentions without citation

Bulbus Fritillariae Cirrhosae as a Respiratory Medicine: Is There a Potential Drug in the Treatment of COVID-19?
Quan et al. (2021)
5 more