› 1f4n

X-ray diffraction
1.9Å resolution

C2 CRYSTAL STRUCTURE OF ALA2ILE2-6, A VERSION OF ROP WITH A REPACKED HYDROPHOBIC CORE AND A NEW FOLD.

Released:
DOI: 10.2210/pdb1f4n/pdb
Model geometry
Fit model/data
PDB entry 1f4n coloured by chain, front view.
PDB entry 1f4n coloured by chain, side view.
PDB entry 1f4n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core.
Willis MA, Bishop B, Regan L, Brunger AT
Structure 8 1319-28 (2000)
PMID: 11188696

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-136490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein rop Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03051 (Residues: 1-63; Coverage: 100%)
Gene name: rop
Sequence domains: Rop protein
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 82.617Å b: 36.488Å c: 47.727Å
α: 90° β: 121.205° γ: 90°
R-values:
R R work R free
0.204 0.204 0.237
Expression system: Escherichia coli

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Citations

4 review citations

Rotamer libraries in the 21st century.
Dunbrack RL. (2002)
3 more

9 mentions without citation

Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard.
Terwilliger et al. (2009)
8 more