› 1f4x

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF AN ANTI-CARBOHYDRATE ANTIBODY DIRECTED AGAINST VIBRIO CHOLERAE O1 IN COMPLEX WITH ANTIGEN

Released:
DOI: 10.2210/pdb1f4x/pdb
PDB entry 1f4x coloured by chain, front view.
PDB entry 1f4x coloured by chain, side view.
PDB entry 1f4x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.
Villeneuve S, Souchon H, Riottot MM, Mazie JC, Lei P, Glaudemans CP, Kovác P, Fournier JM, Alzari PM
Proc Natl Acad Sci U S A 97 8433-8 (2000)
PMID: 10880560

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212997 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ANTIBODY S-20-4, FAB FRAGMENT, LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 22.51 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY S-20-4, FAB FRAGMENT, HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 23.27 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MGS 1 x MGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 45.57Å b: 113.09Å c: 46.24Å
α: 90° β: 100.57° γ: 90°
R-values:
R R work R free
0.185 0.185 0.25

Quick links

Citations

10 review citations

Chemical biology approaches to designing defined carbohydrate vaccines.
Anish et al. (2014)
9 more

4 mentions without citation

Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.
Villeneuve et al. (2000)
3 more