› 1f51

X-ray diffraction
3Å resolution

A TRANSIENT INTERACTION BETWEEN TWO PHOSPHORELAY PROTEINS TRAPPED IN A CRYSTAL LATTICE REVEALS THE MECHANISM OF MOLECULAR RECOGNITION AND PHOSPHOTRANSFER IN SINGAL TRANSDUCTION

Released:
DOI: 10.2210/pdb1f51/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1f51 coloured by chain, front view.
PDB entry 1f51 coloured by chain, side view.
PDB entry 1f51 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
A transient interaction between two phosphorelay proteins trapped in a crystal lattice reveals the mechanism of molecular recognition and phosphotransfer in signal transduction.
Zapf J, Sen U, Hoch JA, Varughese KI
Structure 8 851-62 (2000)
PMID: 10997904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139077 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sporulation initiation phosphotransferase B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 182 amino acids
Theoretical weight: 21.38 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
UniProt:
  • Canonical: P06535 (Residues: 11-192; Coverage: 95%)
Gene names: BSU27930, spo0B, spo0D
Sequence domains:
Structure domains:
Sporulation initiation phosphotransferase F Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 119 amino acids
Theoretical weight: 13.58 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
UniProt:
  • Canonical: P06628 (Residues: 3-121; Coverage: 96%)
Gene names: BSU37130, spo0F
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 72.974Å b: 117.774Å c: 170.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.268
Expression system: Not provided

Quick links

Citations

23 review citations

Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase.
Hengge-Aronis R. (2002)
22 more

34 mentions without citation

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
33 more