› 1f59

X-ray diffraction
2.8Å resolution

IMPORTIN-BETA-FXFG NUCLEOPORIN COMPLEX

Released:
DOI: 10.2210/pdb1f59/pdb
PDB entry 1f59 coloured by chain, front view.
PDB entry 1f59 coloured by chain, side view.
PDB entry 1f59 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
Bayliss R, Littlewood T, Stewart M
Cell 102 99-108 (2000)
PMID: 10929717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162177 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit beta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 442 amino acids
Theoretical weight: 49.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14974 (Residues: 1-442; Coverage: 51%)
Gene names: KPNB1, NTF97
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
FXFG NUCLEOPORIN REPEATS Chains: C, D
Molecule details ›
Chains: C, D
Length: 28 amino acids
Theoretical weight: 2.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 67.246Å b: 211.79Å c: 125.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.262
Expression system: Escherichia coli

Quick links

Citations

77 review citations

Transport into and out of the nucleus.
Macara IG. (2001)
76 more

11 mentions without citation

Floppy but not sloppy: Interaction mechanism of FG-nucleoporins and nuclear transport receptors.
Aramburu et al. (2017)
10 more