› 1f5a

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF MAMMALIAN POLY(A) POLYMERASE

Released:
DOI: 10.2210/pdb1f5a/pdb
PDB entry 1f5a coloured by chain, front view.
PDB entry 1f5a coloured by chain, side view.
PDB entry 1f5a coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of mammalian poly(A) polymerase in complex with an analog of ATP.
Martin G, Keller W, Doublié S
EMBO J 19 4193-203 (2000)
PMID: 10944102

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase alpha Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 59.44 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P25500 (Residues: 1-513; Coverage: 69%)
Gene names: PAP, PAPOLA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 3 x MN
Ligand 3AT 1 x 3AT
Ligand 3PO 1 x 3PO
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 57.55Å b: 62.72Å c: 179.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.261
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
16 more

9 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
8 more