› 1f5s

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF PHOSPHOSERINE PHOSPHATASE FROM METHANOCOCCUS JANNASCHII

Released:
DOI: 10.2210/pdb1f5s/pdb
PDB entry 1f5s coloured by chain, front view.
PDB entry 1f5s coloured by chain, side view.
PDB entry 1f5s coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution.
Wang W, Kim R, Jancarik J, Yokota H, Kim SH
Structure 9 65-71 (2001)
PMID: 11342136

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.63 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58989 (Residues: 1-211; Coverage: 100%)
Gene name: MJ1594
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21212
Unit cell:
a: 69Å b: 69.965Å c: 91.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.235
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
14 more

7 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
6 more