› 1f61

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF ISOCITRATE LYASE FROM MYCOBACTERIUM TUBERCULOSIS

Released:
DOI: 10.2210/pdb1f61/pdb
PDB entry 1f61 coloured by chain, front view.
PDB entry 1f61 coloured by chain, side view.
PDB entry 1f61 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis.
Sharma V, Sharma S, Hoener zu Bentrup K, McKinney JD, Russell DG, Jacobs WR, Sacchettini JC
Nat Struct Biol 7 663-8 (2000)
PMID: 10932251

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 47.21 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKK7 (Residues: 2-428; Coverage: 100%)
Gene names: MTV038.11, Rv0467, icl
Sequence domains: Isocitrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 130.245Å b: 130.245Å c: 288.405Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.165 0.191
Expression system: Escherichia coli

Quick links

Citations

26 review citations

The magic bullets and tuberculosis drug targets.
Zhang Y. (2005)
25 more

15 mentions without citation

Potential inhibitors for isocitrate lyase of Mycobacterium tuberculosis and non-M. tuberculosis: a summary.
Lee et al. (2015)
14 more