› 1fa2

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF BETA-AMYLASE FROM SWEET POTATO

Released:
DOI: 10.2210/pdb1fa2/pdb
PDB entry 1fa2 coloured by chain, front view.
PDB entry 1fa2 coloured by chain, side view.
PDB entry 1fa2 coloured by chain, top view.
Source organism: Ipomoea batatas
Primary publication:
Crystallization, molecular replacement solution, and refinement of tetrameric beta-amylase from sweet potato.
Cheong CG, Eom SH, Chang C, Shin DH, Song HK, Min K, Moon JH, Kim KK, Hwang KY, Suh SW
Proteins 21 105-17 (1995)
PMID: 7777485

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-amylase Chain: A
Molecule details ›
Chain: A
Length: 498 amino acids
Theoretical weight: 56.04 KDa
Source organism: Ipomoea batatas
UniProt:
  • Canonical: P10537 (Residues: 2-499; Coverage: 100%)
Gene names: AMYB, BMY1
Sequence domains: Glycosyl hydrolase family 14
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: RR7, GLC
Carbohydrate polymer
1 bound ligand:
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P42212
Unit cell:
a: 129.63Å b: 129.63Å c: 68.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.281

Quick links

Citations

1 review citation

Beta-amylases from various fungal strains. A review.
Ray RR. (2004)
16 other articles

15 mentions without citation

Comprehensive analyses of the BES1 gene family in Brassica napus and examination of their evolutionary pattern in representative species.
Song et al. (2018)
14 more