› 1fa9

X-ray diffraction
2.4Å resolution

HUMAN LIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH AMP

Released:
DOI: 10.2210/pdb1fa9/pdb
PDB entry 1fa9 coloured by chain, front view.
PDB entry 1fa9 coloured by chain, side view.
PDB entry 1fa9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core.
Rath VL, Ammirati M, LeMotte PK, Fennell KF, Mansour MN, Danley DE, Hynes TR, Schulte GK, Wasilko DJ, Pandit J
Mol Cell 6 139-48 (2000)
PMID: 10949035

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen phosphorylase, liver form Chain: A
Molecule details ›
Chain: A
Length: 846 amino acids
Theoretical weight: 97.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06737 (Residues: 2-847; Coverage: 100%)
Gene name: PYGL
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand GLC 1 x GLC
Ligand AMP 1 x AMP
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 123.91Å b: 123.91Å c: 127.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.235 0.295
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
17 more

7 mentions without citation

Structural Basis for Allostery in PLP-dependent Enzymes.
Tran et al. (2022)
6 more