› 1fay

X-ray diffraction
3.3Å resolution

WINGED BEAN ACIDIC LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE (MONOCLINIC FORM)

Released:
DOI: 10.2210/pdb1fay/pdb
PDB entry 1fay coloured by chain, front view.
PDB entry 1fay coloured by chain, side view.
PDB entry 1fay coloured by chain, top view.
Source organism: Psophocarpus tetragonolobus
Primary publication:
Carbohydrate specificity and salt-bridge mediated conformational change in acidic winged bean agglutinin.
Manoj N, Srinivas VR, Surolia A, Vijayan M, Suguna K
J Mol Biol 302 1129-37 (2000)
PMID: 11183779

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Legume lectin domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 238 amino acids
Theoretical weight: 26.39 KDa
Source organism: Psophocarpus tetragonolobus
UniProt:
  • Canonical: Q9SM56 (Residues: 1-240; Coverage: 99%)
Gene name: wba2
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand AMG 8 x AMG
Ligand MN 8 x MN
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 134.53Å b: 125.91Å c: 138.79Å
α: 90° β: 95.89° γ: 90°
R-values:
R R work R free
0.204 0.204 0.242

Quick links

Citations

14 citation in other articles

Structural basis for the carbohydrate specificities of artocarpin: variation in the length of a loop as a strategy for generating ligand specificity.
Jeyaprakash et al. (2004)
13 more