› 1fc8

Solution NMR

NMR SOLUTION STRUCTURE OF A CHIMERIC OLIGONUCLEOTIDE HAIRPIN R(GGAC)D(TTCG)2'F-A(GTCC)

Released:
DOI: 10.2210/pdb1fc8/pdb
PDB entry 1fc8 coloured by chain, ensemble of 5 models, front view.
PDB entry 1fc8 coloured by chain, ensemble of 5 models, side view.
PDB entry 1fc8 coloured by chain, ensemble of 5 models, top view.
Source organism: Synthetic construct
Primary publication:
NMR solution structure of an oligonucleotide hairpin with a 2'F-ANA/RNA stem: implications for RNase H specificity toward DNA/RNA hybrid duplexes.
Trempe JF, Wilds CJ, Denisov AY, Pon RT, Damha MJ, Gehring K
J Am Chem Soc 123 4896-903 (2001)
PMID: 11457316

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113066 (preferred)
Entry contents:
1 distinct DNA/RNA hybrid molecule
Macromolecule:
5'-R(*GP*GP*AP*CP)D(*TP*TP*CP*GP*(GFL)P*(TAF)P*(CFL)P*(CFL))-3' Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.79 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
3 modified residues:
Ligand GFL 1 x GFL
Ligand TAF 1 x TAF
Ligand CFL 2 x CFL

Experiments and Validation Details

Entry percentile scores
Refinement method: Distance geometry, simulated annealing
Expression system: Not provided

Quick links

Citations

7 review citations

Designing chemically modified oligonucleotides for targeted gene silencing.
Deleavey et al. (2012)
6 more