› 1fcp

X-ray diffraction
2.7Å resolution

FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON

Released:
DOI: 10.2210/pdb1fcp/pdb
PDB entry 1fcp coloured by chain, front view.
PDB entry 1fcp coloured by chain, side view.
PDB entry 1fcp coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Siderophore-mediated iron transport: crystal structure of FhuA with bound lipopolysaccharide.
Ferguson AD, Hofmann E, Coulton JW, Diederichs K, Welte W
Science 282 2215-20 (1998)
PMID: 9856937

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ferrichrome outer membrane transporter/phage receptor Chain: A
Molecule details ›
Chain: A
Length: 705 amino acids
Theoretical weight: 78.52 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06971 (Residues: 52-438, 439-747; Coverage: 98%)
Gene names: JW0146, b0150, fhuA, tonA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GP1, GP4, KDO, GMH, GPH, GLC, GLA
Carbohydrate polymer
6 bound ligands:
Ligand NI 2 x NI
Ligand LIL 2 x LIL
Ligand AAE 1 x AAE
Ligand LIM 1 x LIM
Ligand EA2 1 x EA2
Ligand FCI 1 x FCI
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P61
Unit cell:
a: 171.4Å b: 171.4Å c: 85.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.281
Expression system: Escherichia coli

Quick links

Citations

109 review citations

Lipopolysaccharide endotoxins.
Raetz et al. (2002)
108 more

11 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
10 more