› 1fd7

X-ray diffraction
1.8Å resolution

HEAT-LABILE ENTEROTOXIN B-PENTAMER WITH BOUND LIGAND BMSC001

Released:
DOI: 10.2210/pdb1fd7/pdb
PDB entry 1fd7 coloured by chain, front view.
PDB entry 1fd7 coloured by chain, side view.
PDB entry 1fd7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Exploration of the GM1 receptor-binding site of heat-labile enterotoxin and cholera toxin by phenyl-ring-containing galactose derivatives.
Fan E, Merritt EA, Zhang Z, Pickens JC, Roach C, Ahn M, Hol WG
Acta Crystallogr D Biol Crystallogr 57 201-12 (2001)
PMID: 11173465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat-labile enterotoxin B chain Chains: D, E, F, G, H, L, M, N, O, P
Molecule details ›
Chains: D, E, F, G, H, L, M, N, O, P
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand AI1 10 x AI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21
Unit cell:
a: 60.761Å b: 157.538Å c: 63.145Å
α: 90° β: 116.53° γ: 90°
R-values:
R R work R free
0.187 0.187 0.234
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Heat-labile enterotoxin: beyond G(m1) binding.
Mudrak et al. (2010)
2 more

2 mentions without citation

AB5-Type Toxin as a Pentameric Scaffold in Recombinant Vaccines against the Japanese Encephalitis Virus.
Ahn et al. (2023)
1 more