› 1fdo

X-ray diffraction
2.8Å resolution

OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI

Released:
DOI: 10.2210/pdb1fdo/pdb
PDB entry 1fdo coloured by chain, front view.
PDB entry 1fdo coloured by chain, side view.
PDB entry 1fdo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster.
Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD
Science 275 1305-8 (1997)
PMID: 9036855

Function and Biology Details

Reaction catalysed: 
Formate + an [oxidized hydrogenase] = CO(2) + a [reduced hydrogenase]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase H Chain: A
Molecule details ›
Chain: A
Length: 715 amino acids
Theoretical weight: 79.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07658 (Residues: 1-715; Coverage: 100%)
Gene names: JW4040, b4079, fdhF
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 1 x SF4
Ligand MGD 2 x MGD
Ligand 6MO 1 x 6MO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P41212
Unit cell:
a: 146.6Å b: 146.6Å c: 81.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.281
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
50 more

15 mentions without citation

The mononuclear molybdenum enzymes.
Hille et al. (2014)
14 more