› 1fe8

X-ray diffraction
2.03Å resolution

CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING

Released:
DOI: 10.2210/pdb1fe8/pdb
PDB entry 1fe8 coloured by chain, front view.
PDB entry 1fe8 coloured by chain, side view.
PDB entry 1fe8 coloured by chain, top view.
Source organisms:
Primary publication:
Identification of the collagen-binding site of the von Willebrand factor A3-domain.
Romijn RA, Bouma B, Wuyster W, Gros P, Kroon J, Sixma JJ, Huizinga EG
J Biol Chem 276 9985-91 (2001)
PMID: 11098050

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero octadecamer
PDBe Complex ID:
PDB-CPX-208636 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
von Willebrand factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 196 amino acids
Theoretical weight: 21.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04275 (Residues: 1683-1874; Coverage: 7%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Ig gamma-2A chain C region, A allele Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 210 amino acids
Theoretical weight: 22.42 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01863 (Residues: 1-101; Coverage: 29%)
Gene name: Ighg
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
IMMUNOGLOBULIN IGG RU5 Chains: L, M, N
Molecule details ›
Chains: L, M, N
Length: 211 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand CAC 1 x CAC
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 121.8Å b: 183.6Å c: 131.8Å
α: 90° β: 116.2° γ: 90°
R-values:
R R work R free
0.229 0.227 0.264
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Distribution and evolution of von Willebrand/integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere.
Whittaker et al. (2002)
13 more

11 mentions without citation

Machine Learning Approaches for Proteinā»Protein Interaction Hot Spot Prediction: Progress and Comparative Assessment.
Liu et al. (2018)
10 more