› 1ffz

X-ray diffraction
3.2Å resolution

LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH R(CC)-DA-PUROMYCIN

Released:
DOI: 10.2210/pdb1ffz/pdb
PDB entry 1ffz coloured by chain, front view.
PDB entry 1ffz coloured by chain, side view.
PDB entry 1ffz coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis of ribosome activity in peptide bond synthesis.
Nissen P, Hansen J, Ban N, Moore PB, Steitz TA
Science 289 920-30 (2000)
PMID: 10937990

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113067 (preferred)
Entry contents:
1 distinct RNA molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 602 nucleotides
Theoretical weight: 194.85 KDa
Source organism: Haloarcula marismortui
Sequence domains: Archaeal large subunit ribosomal RNA
R(P*CP*C*)-D(P*A)-R(P*(PU)) Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PU 1 x PU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 212Å b: 300Å c: 574Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

336 review citations

How antibiotics kill bacteria: from targets to networks.
Kohanski et al. (2010)
335 more

7 mentions without citation

POPSCOMP: an automated interaction analysis of biomolecular complexes.
Kleinjung et al. (2005)
6 more