› 1fg2

X-ray diffraction
2.75Å resolution

CRYSTAL STRUCTURE OF THE LCMV PEPTIDIC EPITOPE GP33 IN COMPLEX WITH THE MURINE CLASS I MHC MOLECULE H-2DB

Released:
DOI: 10.2210/pdb1fg2/pdb
PDB entry 1fg2 coloured by chain, front view.
PDB entry 1fg2 coloured by chain, side view.
PDB entry 1fg2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Viral escape at the molecular level explained by quantitative T-cell receptor/peptide/MHC interactions and the crystal structure of a peptide/MHC complex.
Tissot AC, Ciatto C, Mittl PR, Grütter MG, Plückthun A
J Mol Biol 302 873-85 (2000)
PMID: 10993729

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134996 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 281 amino acids
Theoretical weight: 32.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-304; Coverage: 83%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
LCMV PEPTIDIC EPITOPE GP33 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 9 amino acids
Theoretical weight: 1.02 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P21
Unit cell:
a: 92.527Å b: 124.77Å c: 99.555Å
α: 90° β: 103.03° γ: 90°
R-values:
R R work R free
0.236 0.236 0.276
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural and thermodynamic correlates of T cell signaling.
Rudolph et al. (2002)
2 more

10 mentions without citation

Modeling the structure of bound peptide ligands to major histocompatibility complex.
Tong et al. (2004)
9 more