› 1fgs

X-ray diffraction
2.4Å resolution

FOLYLPOLYGLUTAMATE SYNTHETASE FROM LACTOBACILLUS CASEI

Released:
DOI: 10.2210/pdb1fgs/pdb
PDB entry 1fgs coloured by chain, front view.
PDB entry 1fgs coloured by chain, side view.
PDB entry 1fgs coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Structural homologies with ATP- and folate-binding enzymes in the crystal structure of folylpolyglutamate synthetase.
Sun X, Bognar AL, Baker EN, Smith CA
Proc Natl Acad Sci U S A 95 6647-52 (1998)
PMID: 9618466

Function and Biology Details

Reaction catalysed: 
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.65 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli
UniProt:
  • Canonical: P15925 (Residues: 1-428; Coverage: 100%)
Gene name: fpgS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 54Å b: 46.1Å c: 84.9Å
α: 90° β: 107.3° γ: 90°
Expression system: Escherichia coli

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Citations

7 review citations

Molecular basis of antifolate resistance.
Assaraf YG. (2007)
6 more

5 mentions without citation

Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin.
Kitadokoro et al. (2007)
4 more