1fh8

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF THE XYLANASE CEX WITH XYLOBIOSE-DERIVED ISOFAGOMINE INHIBITOR

Released:

Function and Biology Details

Reactions catalysed:
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase/xylanase Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.05 KDa
Source organism: Cellulomonas fimi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07986 (Residues: 42-353; Coverage: 70%)
Gene names: cex, xynB
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 85.31Å b: 85.31Å c: 79.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.224
Expression system: Escherichia coli