› 1fhw

X-ray diffraction
1.9Å resolution

Structure of the pleckstrin homology domain from GRP1 in complex with inositol(1,3,4,5,6)pentakisphosphate

Released:
DOI: 10.2210/pdb1fhw/pdb
PDB entry 1fhw coloured by chain, front view.
PDB entry 1fhw coloured by chain, side view.
PDB entry 1fhw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains.
Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA
Mol Cell 6 373-84 (2000)
PMID: 10983984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-126701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytohesin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 15.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08967 (Residues: 264-391; Coverage: 32%)
Gene names: Cyth3, Grp1, Pscd3
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:
Ligand I5P 2 x I5P
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 37.97Å b: 72.22Å c: 46.9Å
α: 90° β: 93.21° γ: 90°
R-values:
R R work R free
0.229 0.229 0.27
Expression system: Escherichia coli

Quick links

Citations

44 review citations

The protein kinase B/Akt signalling pathway in human malignancy.
Nicholson et al. (2002)
43 more

13 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
12 more