› 1fif

X-ray diffraction
1.95Å resolution

N-ACETYLGALACTOSAMINE-SELECTIVE MUTANT OF MANNOSE-BINDING PROTEIN-A (QPDWG-HDRPY)

Released:
DOI: 10.2210/pdb1fif/pdb
PDB entry 1fif coloured by chain, front view.
PDB entry 1fif coloured by chain, side view.
PDB entry 1fif coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
Feinberg H, Torgersen D, Drickamer K, Weis WI
J Biol Chem 275 35176-84 (2000)
PMID: 10931846

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannose-binding protein A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 154 amino acids
Theoretical weight: 17.19 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19999 (Residues: 90-197, 199-206, 207-238; Coverage: 67%)
Gene name: Mbl1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

2 bound ligands:
Ligand CA 9 x CA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 80.34Å b: 84.833Å c: 97.142Å
α: 90° β: 107.88° γ: 90°
R-values:
R R work R free
0.193 0.193 0.229
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The C-type lectin-like domain superfamily.
Zelensky et al. (2005)
8 more

2 mentions without citation

Scavenger receptor C-type lectin binds to the leukocyte cell surface glycan Lewis(x) by a novel mechanism.
Feinberg et al. (2007)
1 more