› 1fim

X-ray diffraction
2.2Å resolution

MACROPHAGE MIGRATION INHIBITORY FACTOR

Released:
DOI: 10.2210/pdb1fim/pdb
PDB entry 1fim coloured by chain, front view.
PDB entry 1fim coloured by chain, side view.
PDB entry 1fim coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of the macrophage migration inhibitory factor from rat liver.
Suzuki M, Sugimoto H, Nakagawa A, Tanaka I, Nishihira J, Sakai M
Nat Struct Biol 3 259-66 (1996)
PMID: 8605628

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.28 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P30904 (Residues: 2-115; Coverage: 99%)
Gene name: Mif
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
Spacegroup: P63
Unit cell:
a: 61.8Å b: 61.8Å c: 53.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.244
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Macrophage migration inhibitory factor: a regulator of innate immunity.
Calandra et al. (2003)
29 more

4 mentions without citation

A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1.
Wang et al. (2016)
3 more