› 1fir

X-ray diffraction
3.3Å resolution

CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTION PRIMER TRNA(LYS3)

Released:
DOI: 10.2210/pdb1fir/pdb
PDB entry 1fir coloured by chain, front view.
PDB entry 1fir coloured by chain, side view.
PDB entry 1fir coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The crystal structure of HIV reverse-transcription primer tRNA(Lys,3) shows a canonical anticodon loop.
Bénas P, Bec G, Keith G, Marquet R, Ehresmann C, Ehresmann B, Dumas P
RNA 6 1347-55 (2000)
PMID: 11073212

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195823 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
HIV-1 REVERSE TRANSCRIPTION PRIMER TRNA(LYS3) Chain: A
Molecule details ›
Chain: A
Length: 76 nucleotides
Theoretical weight: 24.85 KDa
Source organism: Bos taurus
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
9 modified residues:
Ligand 2MG 2 x 2MG
Ligand H2U 3 x H2U
Ligand PSU 3 x PSU
Ligand 70U 1 x 70U
Ligand 12A 1 x 12A
Ligand 7MG 1 x 7MG
Ligand 5MC 2 x 5MC
Ligand 2MU 1 x 2MU
Ligand 1MA 1 x 1MA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P3212
Unit cell:
a: 100.72Å b: 100.72Å c: 82.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.227

Quick links

Citations

8 review citations

Post-transcriptional nucleotide modification and alternative folding of RNA.
Helm M. (2006)
7 more

24 mentions without citation

Wobble uridine modifications-a reason to live, a reason to die?!
Schaffrath et al. (2017)
23 more