› 1fix

X-ray diffraction
2.3Å resolution

THE STRUCTURE OF AN RNA/DNA HYBRID: A SUBSTRATE OF THE RIBONUCLEASE ACTIVITY OF HIV-1 REVERSE TRANSCRIPTASE

Released:
DOI: 10.2210/pdb1fix/pdb
PDB entry 1fix coloured by chain, front view.
PDB entry 1fix coloured by chain, side view.
PDB entry 1fix coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The structure of an RNA/DNA hybrid: a substrate of the ribonuclease activity of HIV-1 reverse transcriptase.
Horton NC, Finzel BC
J Mol Biol 264 521-33 (1996)
PMID: 8969302

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113804 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
DNA (5'-D(*GP*GP*CP*GP*CP*CP*CP*GP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*UP*UP*CP*GP*GP*GP*CP*GP*CP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.17 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4322
Unit cell:
a: 31.3Å b: 31.3Å c: 137.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.145 not available
Expression system: Not provided

Quick links

Citations

9 review citations

Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition.
Sarafianos et al. (2009)
8 more

6 mentions without citation

Refinement of the AMBER force field for nucleic acids: improving the description of alpha/gamma conformers.
Pérez et al. (2007)
5 more