› 1fjr

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE ECTODOMAIN OF METHUSELAH

Released:
DOI: 10.2210/pdb1fjr/pdb
PDB entry 1fjr coloured by chain, front view.
PDB entry 1fjr coloured by chain, side view.
PDB entry 1fjr coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-coupled receptor associated with extended lifespan.
West AP, Llamas LL, Snow PM, Benzer S, Bjorkman PJ
Proc Natl Acad Sci U S A 98 3744-9 (2001)
PMID: 11274391

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
G-protein coupled receptor Mth Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.52 KDa
Source organism: Drosophila melanogaster
Expression system: unidentified baculovirus
UniProt:
  • Canonical: O97148 (Residues: 25-219; Coverage: 40%)
Gene names: CG6936, mth
Sequence domains: Methuselah N-terminus
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 5 x NAG
Ligand SO4 2 x SO4
Ligand PB 4 x PB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 68.937Å b: 73Å c: 115.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.227
Expression system: unidentified baculovirus

Quick links

Citations

6 review citations

Sequence analyses of G-protein-coupled receptors: similarities to rhodopsin.
Mirzadegan et al. (2003)
5 more

6 mentions without citation

Activation of G protein-coupled receptors: beyond two-state models and tertiary conformational changes.
Park et al. (2008)
5 more