› 1fkq

X-ray diffraction
1.8Å resolution

RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V

Released:
DOI: 10.2210/pdb1fkq/pdb
PDB entry 1fkq coloured by chain, front view.
PDB entry 1fkq coloured by chain, side view.
PDB entry 1fkq coloured by chain, top view.
Source organism: Capra hircus
Primary publication:
Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.
Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I
Proteins 45 16-29 (2001)
PMID: 11536356

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + D-glucose = UDP + lactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-lactalbumin Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.34 KDa
Source organism: Capra hircus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00712 (Residues: 20-142; Coverage: 100%)
Gene name: LALBA
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 42.75Å b: 59.38Å c: 45.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

α-Lactalbumin, Amazing Calcium-Binding Protein.
Permyakov EA. (2020)
5 other articles

1 mention without citation

Using SAD data in Phaser.
Read et al. (2011)